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Monitoring of mesenchymal stem cell migration in the extracellular matrix
Zumberg, Inna ; Čmiel, Vratislav (referee) ; Chmelíková, Larisa (advisor)
This master’s thesis contains a description of mesenchymal stem cells (MSC). The work includes knowledge about the process of migration and differentiation of MSCs. The theoretical part of this thesis also deals with the problem of cell cultivation in 2D and 3D environments. The most used materials for creating 3D scaffolds are described here. The practical part contains the description of the cell culture protocol for passaging and also describes the experiment in the cell laboratory. The results of the experiment are discussed using confocal microscope images. The proposed experiment was tested with a sufficient number of repetitions. The processing of microscopic images was performed in the MATLAB programming environment.
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Viability evaluation on cultivated mezenchymal cells
Jehličková, Jana ; Odstrčilík, Jan (referee) ; Čmiel, Vratislav (advisor)
This thesis deals with the possibilities of cell viability determination and its evalution. The thesis in theory describes cell cultivation, different methods of staining cells and display options. In the practical part is mentioned the preparation of the observed experiment and there are shown the examples of cell images created by confocal microscopy. Additionaly there are created the data analysis algorithm in Matlab. For setting suitable analysis parameters is formed user interface.
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Evaluation of antibiotic-induced mitochondrial superoxide production in adherent cells
Ingrová, Kateřina ; Chmelíková, Larisa (referee) ; Zumberg, Inna (advisor)
The theoretical part of this bachelor´s thesis contains a description of the effect of reactive oxygen species on oxidative stress by mitochondria and the consequences of antibiotics use in cell line culturing. The cell line studied in this bachelor´s thesis is the mesenchymal stem cells (MSCs). The practical part describes the procedure of the experiment including cell culturing, passaging and cell labeling. The proposed experiment was repeated with sufficient number of repetitions. Finally, confocal microscopy images were processed in the MATLAB programming environment.
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Evaluation of antibiotic-induced mitochondrial superoxide production in adherent cells
Ingrová, Kateřina ; Chmelíková, Larisa (referee) ; Zumberg, Inna (advisor)
The theoretical part of this bachelor´s thesis contains a description of the effect of reactive oxygen species on oxidative stress by mitochondria and the consequences of antibiotics use in cell line culturing. The cell line studied in this bachelor´s thesis is the mesenchymal stem cells (MSCs). The practical part describes the procedure of the experiment including cell culturing, passaging and cell labeling. The proposed experiment was repeated with sufficient number of repetitions. Finally, confocal microscopy images were processed in the MATLAB programming environment.
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Nanostructured Modifcation of Material Surfaces for Controlled Cell Cultivation
Kotelnikov, Ilya ; Proks, Vladimír (advisor) ; Chytil, Petr (referee) ; Šebestová Janoušková, Olga (referee)
Commercial media and surfaces for cell cultivation do not promote conditions for cell cultivation and proliferation with specific interactions cell-material surface. The aim of this thesis is developing surfaces for cell cultivation which mimic conditions in living tissues. The approach introduced in this thesis is based on applying biomimetic peptide ligands on inert supporting materials with non-fouling properties. Considering that a choice of a ligand sequence and distance between peptides can dramatically influence the outcome, a few model peptides with varying parameters were synthesized and investigated. The cell adhesive peptides were synthesized by solid phase peptide synthesis and scrambled peptides were synthesized and tested as well. Spatial deposition of peptides is another highly important point of study. The 'click'- reaction was used to successfully immobilize the peptides on produced surfaces. The distance between the peptide molecules on the surfaces was controlled by their concentration in a reaction mixture. The reference samples were immobilized with radiolabeled peptides for quantitative estimation of the peptides present. Then, the materials with different types of peptides and range of concentrations were examined via cultivation of cell cultures. The experiments were focused on...
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Monitoring of mesenchymal stem cell migration in the extracellular matrix
Zumberg, Inna ; Čmiel, Vratislav (referee) ; Chmelíková, Larisa (advisor)
This master’s thesis contains a description of mesenchymal stem cells (MSC). The work includes knowledge about the process of migration and differentiation of MSCs. The theoretical part of this thesis also deals with the problem of cell cultivation in 2D and 3D environments. The most used materials for creating 3D scaffolds are described here. The practical part contains the description of the cell culture protocol for passaging and also describes the experiment in the cell laboratory. The results of the experiment are discussed using confocal microscope images. The proposed experiment was tested with a sufficient number of repetitions. The processing of microscopic images was performed in the MATLAB programming environment.
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Viability evaluation on cultivated mezenchymal cells
Jehličková, Jana ; Odstrčilík, Jan (referee) ; Čmiel, Vratislav (advisor)
This thesis deals with the possibilities of cell viability determination and its evalution. The thesis in theory describes cell cultivation, different methods of staining cells and display options. In the practical part is mentioned the preparation of the observed experiment and there are shown the examples of cell images created by confocal microscopy. Additionaly there are created the data analysis algorithm in Matlab. For setting suitable analysis parameters is formed user interface.
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